ABSTRACT: Protease is one of
the widely used enzymes for the industry. The potential resource of
microorganism that produced protease is milk cow waste. In this research, isolation
and characterization has been done toward isolated protease from milk cow waste
of the Exfarm’s Animal Husbandry Faculty at University of Jenderal Soedirman,
Purwokerto. The research used experiment method and the parameters observed
were the genus of bacteria which produce protease and the activity of protease.
The characterizations of protease were determination of optimum pH and
temperature, the influence of metal ions, EDTA, surfactant, and commercial
detergent toward enzyme activity, and also the study of enzyme stability. The
results from the research showed that the isolated bacteria from the Exfarm’s
of Animal Husbandry Faculty of UNSOED, which produced protease was Salmonella
sp. Characterization of isolated Salmonella sp. from 45% ammonium sulphate
fraction indicated that the optimum temperature was 50 ºC, optimum pH was 8,
the enzyme was activated by Ca2+ dan Mg2+ ion, whereas it was inhibited by
Zn2+, Cu2+ ions and EDTA. The addition of Tween-80 with the concentration of
0.2% and 0.4% increased protease activity, however the addition of Tween-80
with concentration higher than 0.6% decreased the protease activity. Enzyme
protease from isolated Salmonella sp. was relatively stable with the addition
of commercial detergent such as Attack, Surf, and Bukrim.
Keyword: Protease, milk cow
waste, detergent industry
Penulis: Zusfahair, Puji
Lestari, Ari Asnani
Kode Jurnal: jpkimiadd110037
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