ABSTRACT: Yeast Saccharomyces
cerevisiae is an excellent model to studi genes function of eukarotic cells
such as study of gene encoding protein phosphatase PTC2. Novel phenotypic
caused by mutated gene is an important step to study function of gene. In this
study constructed mutant of PTC2 gene encoding protein phosphatase. Method that
used in this construction was replacement of target gene (PTC2) with auxotroph
marker Candida albicans HIS3 by Polymer Chain Reaction (PCR) or called by
PCR-mediated disruption. Mutant colonies which grew in selective medium SC
without histidine were confirmed by PCR amplification. By using 1% Agarose gel
electrophoresis the result showed that size of ptc2D::CgHIS3 transformant was
3.52 kb while wild type strain was 2.9 kb, indicated that ptc2D::CgHIS3 has
integrated on chromosome V replacing PTC2 wild type.
Keywords: PTC2, Saccharomyces
cereviciae, PCR, single disruptant
Penulis: Hermansyah
Kode Jurnal: jpkimiadd110042
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