OPTIMASI KONSENTRASI INOKULUM, RASIO C:N:P DAN pH PADA PROSES BIOREMEDIASI LIMBAH PENGILANGAN MINYAK BUMI MENGGUNAKAN KULTUR CAMPURAN
ABSTRAK: The purposes of this
research were to obtain the best inoculum concentration, C:N:P ratio, and pH,
and also to identify the ability of mixed culture of hydrocarbonoclastic
bacteria in oil waste degradation.
The isolats were
used are Acinetobacter
baumanni, Alcaligenes eutrophus, Bacillus
sp1., Methylococcus capsulatus,
Bacillus sp2., Morococcus
sp., Pseudomonas diminuta, Xanthomonas
albilineans, Bacillus cereus
and Flavobacterium branchiophiia. Variation
of inoculum concentrations were
10%, 15%, and
20% (v/v), C:N:P ratios were
100:10:1, 100:10:0,5, 100:5:1, and 100:5:0,5, and pH were 6,5, 7,0, 7,5. Observed parameters
in optimization were
Total Plate Count
(TPC) the culture
every 24 hours, Total
Petroleum Hydrocarbon (TPH)
and Chemical Oxygen
Demand (COD) examined at
the end of
the bioremediation period.
Best optimization result
then analyzed with GC/MS.
Optimization result indicated the best inoculum concentration was 10% with TPH
degradation 61,79% and COD slope 61,75%. It is assumed that the low value of
TPH degradation and COD
slope at 15%
and 20% inoculum
concentration were caused
by competition inside the
bacterial population at
that high inoculum
concentration. The competition result
in low growth
and degradation. C:N:P
ratio was 100:5:1
with TPH degradation 66,55% and
COD slope 85,18%. It
is assumed that
the C:N:P ratio
is equal, so it can enhance the
bioremediation procces. The best pH was 7,5 with TPH degradation 73,24% and COD
slope 86,28%. The process at the optimum conditions using inoculum as a mixed
culture enhanced the
bioremediation process with
the result as
follows, TPH degradation 93,06%,
COD 90,73% for
treatment. The chromatogram
indicated that total hydrocarbon compound
from nC9 – nC32 have
been degraded by
43,413% – 63,117%.
A good result of
bioremediation was obtained
from mixed culture
inoculum at 10% concentration, C:N:P ratio of 100:5:1,
and pH 7,5.
Keywords: Bioremediation, hydrocarbonoclastic bacteria,
inoculum concentration, C:N:P
ratio, pH
Penulis: Syukria Ikhsan Zam
Kode Jurnal: jpbiologidd100009
