PENGARUH PEMBERIAN PROBIOTIK Pediococcus pentosaceus ISOLAT DADIH SEBAGAI ANTI DIARE PADA MENCIT PUTIH (Mus muscullus)

Abstract: Pediococcus pentosaceus isolated from curd was performed as anti diarrhea in white mice (Mus muscullus). Curd was got from Lintau, Batusangkar, West Sumatra. Probiotic are able to suppress the growth of pathogenic bacteria in the intestine which cause diarrhea. Probiotics produce antimicrobial substances such as lactic acid, bacteriocins, H2O2, etc.,which can suppress the growth of pathogenic bacteria. The bowel frequence, microflora balance and high of ileum villus were measured. The result of this experiment showing there was different significant interaction (p<0,01) between dose supplementation with treatment time on the bowel frequence, microflora balance and the high of ileum villus.

The results of this experiment was obtained mean defecation frequency at 55 on A2B2 treatment by supplementation EPEC after 12 hours and without adding Pediococcus pentosaceus. The highest mean of LAB total colony goes to the A3B2 treatment by supplementation EPEC and Pediococcus pentosaceus 1x108 CFU/ml after 12 hours of 97 x 107 CFU/g. The highest mean of Escherichia coli total colony goes to A2B3 treatment by supplementation EPEC after 24 hours and without adding the probiotics at 21,5 x 107 CFU/g. And the highest villus goes to A3B2 treatment at 374,51 µm.

Keywords: Curd Dadih, Pediococcus pentosaceus, Diarrhea

Penuli: Rina Handayani, Sumaryati Syukur, dan Endang Purwati

Kode Jurnal: jpkimiadd120010

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