SELEKSI BAKTERI PENAMBAT NITROGEN DAN PENGHASIL HORMON IAA (Indole Acetic Acid) DARI RIZOSFER TANAH PERKEBUNAN KEDELAI (Glycine max L.)
Abstract: Rizosphere bacteria
can be used to fix nitrogen and produce
IAA (Indol Acetic Acid) as biofertilizer to support plant growth. The ability
of rhizosphere bacteria can be improved using biotechnology. The aim of this
study is to select the
most potential nitrogen
fixing and IAA
producing bacteria. Nitrogen
fixing and IAA
producing bacteria were isolated using JNFB and Luria Bertani +
L-tryptofan medium respectively. The ability of nitrogen fixing bacteria
were tested by ARA method,
while the ability
of IAA producing
bacteria were investigated
by spectrophotometer technique of 535 nm. The highest IAA concentration was produced by isolate I3
which was 33.3 ppm and
the highest concentration
of nitrogen was
yielded by isolate
N3 which was
29.93 ppm. Both N3
isolate and I3 isolate potentially as biofertilizer known as PGPR
(Plant Growth Promoting Rhizobacteria).
Keywords: nitrogen fixing bacteria,
IAA producing bacteria, PGPR
Penulis: Ratna Sari Tarigan,
It Jamilah dan Elimasni
Kode Jurnal: jpbiologidd130042
