ABSTRACT: The aim of this
research is to find the method for analyze glimepiride and it’s metabolite.
Glimepiride is the second generation of antidiabetic oral from the sulphonyl urea
that works by stimulating the insulin secretion from beta cells of pancreas. Glimepiride
is isolated from plasma the using chloroform. Using the high performance liquid
chromatography method which include C18 reversed phase column, using
mixture of methanol:water (50:50, v/v) as a mobile phase, flow rate 1.0 ml/ minutes,
detection at wave lenght 228 nm with photo diode array detector gives retention
times of glimepiride in 17 minutes without any interference from endogen component
of plasma and from it’s metabolite. Linearity with added internal standard gliclazide
was established for the range concentration 100-1000 ng/ml with coefficient of
correlation (r) is 0.9977 and give the limit of quantitation of glimepiride in 50
ng/ml. The results of validation method fulfilled for the given criterias.
Key Words: Glimepiride, blood
plasma, HPLC
Penulis: Yahdiana Harahap,
Umar Mansur, Theresia Sinandang
Kode Jurnal: jpfarmasidd060013
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