ABSTRACT: A research on the
ability of protein fraction from red
algae Gelidium amansii to inhibit the growth of
Salmonella thypi and Staphylococcus aureus has been conducted. Proteins
was fractionated from the crude extract by salting out method with 0 – 20 %, 20 – 40 %, 40 – 60 % and 60 – 80 %
ammonium sulfat saturation. Proteins was purified by a dialysis method using a
selophan membrane. The protein level was determined by Lowry’s method, the
highest protein concentration, 5.6 mg/mL was found in the 40 – 60 % fraction of the red algae. Antibacterial activity test
was performed using a layered jelly diffusion method on the MHA medium.
Results indicated that
crude extract and
the protein fraction
of the red
algae had antibacterial effect
to Salmonella thypi
and Staphylococcus aureus.
The highest antibacterial
activity to Staphylococcus aureus
was found in the 0-20%
fraction with the
inhibition diameter of
14.43 mm after incubated for 24 hours. These research
might provide a significant results for the following research on the antibacterial
action in molecular level of protein
compounds from marine algae.
Keywords: antibacterial,
Lowry’s method, protein fraction, red algae
Penulis: Seniwati Dali, Hasnah
Natsir, Hanapi Usman dan Ahyar Ahmad
Kode Jurnal: jpfarmasidd110015
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