ABSTRACT: Custard apple
(Annona squamosa L.) is one of efficacious medical plant that often used by
local community for constipation, insectiside, carbuncle, scabies,
gastrointestinal problem such diarrhoea, dysentery and anthelmintic. In
Indonesia, worm disease is infection disease that still high prevalention.
Although modern anthelmintic is in stock, but still common community is to use
medical plant, specialy custard apple for therapeutic worm disease. The aim of
this research was to deterinate anthelmintic capability of ethanol extract of
seed custard apple on the worm Ascaridia galli on in vitro. This researches
were pure experimental by using Lamson and Brown method submerges that have
been modified by using 270 of worm femele, that devided into 9 groups treatment,
which was 4 groups for ethanol extract of seed custerd apple in concentration
of 10 mg/mL, 20 mg/mL, 30 mg/mL, 40 mg/mL, 4 positive control groups (citrate
piperazinae 2 mg/mL, 4 mg/mL, 6 mg/mL, 8 mg/mL) and negative control group
(NaCl 9 mg/mL). Each group consist of 6 petri disc and each petri disc contain
5 of worm and 25 mL solution. Anthelmintic capability is measurement observed
is the mortality time rate of the worm and value LC50, that is concentration is
needed for killing 50% worm population and than analysed date by one way ANOVA
and continued by Least Significant Difference (LSD) test. The research result,
shown that mortality time rate of worm after submerges by ethanol extract of
seed custrad apple in concentration 10 mg/mL, 20 mg/mL, 30 mg/mL, and. 40 mg/mL
is 43,33; 38,00; 37,66 and 34,33 hours. Exactly after submerges by citrate
piperazinae 4 mg/mL shown mortality time rate of worm is 34,33 hour. LC50 value
of ethanol extract of seed custard apple and piperazinae citrate at 12th hour is
36,94 mg/mL and 2,26 mg/mL. Result Thin Layer Chromatography (TLC) shown seed
custard apple containes compounds such as from alkaloid, flavonoid and tanin.
That compounds is posibility efficacious anthelmintic.
Keywords: Annona squamosa L.,
anthelmintic, Lamson and Brown method submerges
Penulis: Riyanta aribawa, Atiq
fauziyah, Mustofa mustofa
Kode Jurnal: jpfarmasidd080019
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