ABSTRACT: Alfalfa plant
(Medicago sativa L.) contains of flavonoids compound, it has already known. One
of the effects of flavonoids is anti-inflammatory effect of alfalfa herb (M.
sativa L.). The purpose of this research to know about anti-inflammatory effect
of alfalfa herb ethanolic extract on carragenan induced male rats and also to
know the content of its active compound. Thirty male wistar rats were randomly
divided into 5 group of 6. Group I, negative control, were treathed CMC-Na 0,5
%, group II, positive control, were treathed natrium diclofenac 6,3 mg/ kg BB.
Group III, IV and V were treathed ethanolic extract of alfalfa herb orally dose
45 mg/ kg BW, 90 mg/ kg BW and 180 mg/ kg BW. Anti-inflammatory effect were
measured of edema feet were done every 30 minutes for 6 hours using
plestismometer. After edema feet data vs time got, then we can get AUC0-360
value and percentase anti-inflammatory effect. Statistic analytical used one
way ANOVA (p<0,05) and continued with Tukey test then. Identification of active
compound of alfalfa herb ethanolic extract was used Thiny Layer Chromatography
(TLC). The result of the research shown that ethanolic extract of alfalfa herb
by dosage 45 mg/ kg BW, 90 mg/ kg BW and 180 mg/ kg BW could reduce edema in
carrageenan induced rats, significantly. The percentase of anti-inflammatory
effect on dosage of 45 mg/ kg BW is 58,99 %, on 90 mg/ kg BW is 72,64 % and on
180 mg/ kg BW is 64,23 %. TLC result shown that active compound the ethanolic
extract of alfalfa herbs was flavonoids. We could be concluded that ethanolic
extract of alfalfa herb have anti-inflammatory effect.
Key word: ethanolic extract of
alfalfa herb (Medicago sativa L.), flavonoids, anti-inflammatory
Penulis: Sri susilowati,
yuniarti rosyidah
Kode Jurnal: jpfarmasidd080023
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