ABSTRACT: Tradisional herbs
made from natural ingredients containing many organic elements as the material
feed for microbe that caused self decompose during storage. On the previously
result of research did by Subhan Ratnawati (2002) shows there were many bacteria,
including pathogen bacteria on galian singset herbal tonic carried in
Jogjakarta city. The purpose study was intended to find out quantities and
identified of bacteria and fungus present in the herbal tonic at ten regions in
southern Semarang District. This study was conducted using 52 samples of „beras
kencur and temulawak herbs‟ sold in Southern Semarang District. ALT method was
used on this experiment and the medium used for growing in an aerobic manner
was PCA (Plate Count Agar), while that used for growing in an anaerobic
condition were TSA (Tripticase Soya Agar) and was incubated at 37oC for 24
hours. The media used for fungi were PDA (Potato Dextrose Agar). We used a
100-times amplifying microscope for identifying the fungi. The analysis on the
data were conducted by calculating the numbers of bacteria and fungi for each
sample and by identifying the bacteria and fungi present in the herbs. These
numbers then compared to the prerequisites contained in the Indonesian Health
Office (MENKES RI) No. 661/MENKES/SK/VII/1994. The analysis showed that the
average number of bacteria in the sample of „beras kencur and temulawak herbs‟
that grown at the aerobic media was 4.7x105 CFU/ml and 3.1x104 CFU/ml, but we
found no bacteria in the sample grown in an anaerobic manner. The numbers of
bacteria grown in the aerobic manner exceeded the upper limits for bacteria
number 104 (10000 CFU/ml). The average number of fungi from all samples was
50.7 CFU/ml and 40.5 CFU/ml, which was below the standard of 103 (1000 CFU/ml).
The aerobic bacteria found in this study were Pseudomonas aeruginosa and
Eschericia coli while the fungi found were Rhizopus, Aspergillus niger,
Moniliaceae and Penisillium. Nearly all samples that examined in this study did
not meet the conditions stipulated by Indonesian Health Office (MENKES RI)
Number 661/MENKES/SK/VII/1994.
Keywords: Herbal tonic
carried, Bacteria Number and Fungi Number
Penulis: Maulita cut nuria,
Dedeh ratna dewi, Septaningsih septaningsih, Sumantri sumantri
Kode Jurnal: jpfarmasidd080025
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