ABSTRACT: In medicine,
polyphenol oxidases are used
for prevention of bacterial
adhesion, treatment of Parkinson’s
disease and control of melanin synthesis. Polyphenol oxidase could be produce
by endophytic fungus from podhusk of Theobroma cacao. To produce microbial
enzyme, media composition should be optimized. The aim of this study was to investigate
the effect of cupri sulphate concentration as inducer in fermentation
medium containing 1 % yeast extract, 0,05 % MgSO4, 0.1% KH2PO4,
0,051 % gallic acid, and CuSO4.5H2O 0-50
ppm. The result
showed that it was
effect addition of CuSO4
in fermentation medium toward production of polyphenol oxidase. Optimal
concentration of CuSO4 to produce polyphenol oxidase was 25 bpj with
enzyme activity 530 U/mL using catechol
as substrate.
Keywords:
endophytic fungus, cocoa podhusk, cupri sulphate, polyphenol oxidase
Penulis: Sartini , Abd.Rauf
Patong, Tjodi Harlim, dan Pirman
Kode Jurnal: jpfarmasidd110006
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