VALIDASI METODE SPEKTROFOTOMETRI SINAR TAMPAK UNTUK ANALISIS FORMALIN DALAM TAHU

ABSTRACT: The research about validation of visible spectrophotometry method to analyze formalin in tofu had been  carried  out.  The  aim  of  this  research  was  to  determine  several  validation  parameters  such  as precision,  linearity,  accuracy,  Limit  of  detection  (LOD)  and  Limit  of  quantification  (LOQ).  Formalin  was added  Nash  reagent  then  detected  using  spectrophotometer  at  wave  length  412  nm.The  results  of  the research showed that validation parameters values for accuracy was 102,84%; standard deviation value for formalin was 1,198%; linearity value for formalin was 0,9994; LOD was 0,0375 μg/mL and LOQ was 0,1250 μg/mL, respectively. Due to the result of the research, visible spectrophotometry method to analyze formalin in tofu was valid.
Key words: Validation, spectrophotometry, LOQ, LOD
Penulis: Muhammad Aswad, Aisyah Fatmawaty, Nursamsiar, dan Rahmawanti
Kode Jurnal: jpfarmasidd110019

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