AKTIVITAS ANTIOKSIDAN DEDAK SORGUM VARIETAS COKLAT (Sorghum bicolor) HASIL EKSTRAKSI BERBAGAI PELARUT
ABSTRACT: A completely randomized
design experiment was conducted to determine the antioxidant activity of bran
extracts obtained by applying various solvents to the local brown sorghum
grain. Four types of technical grade of solvents were employed, namely
aquadest, ethanol 96%, methanol 98% and acetone 98%. Each bran extract was
obtained by shaking for 8 hrs in a single solvent, respectively. The extract
was vacuum dried prior to use. The antioxidant activity of the extract was
assessed by determining its ability for free radicals (DPPH) scavenging and for
the ability for oxidation inhibition of a particular fatty acid with ferric
thiocyanate.The results indicated that the levels of phenols, tannin and
anthocyanin, varied with the solvent and the methanol extract contained the
highest level of total phenols (55.7%). The highest radical scavenging activity
was found at a concentration of 400 ppm. The methanol extract (400 ppm) showed
the highest EC50 value (81,5 %), which was slightly lower than that of BHT. The
order of oxidative inhibition, from the highest one, was the extracts of
methanol > ethanol > acetone > aquadest. The value of the methanol
extract was almost comparable the one of BHT. Both the scavenging activity and
oxidative inhibition were correlated positively with the levels of tannin and
other phenols, but negatively correlated with the anthocyanin content.
Keywords: brown sorghum, bran
extracts, antioxidant activity
Penulis: Juwita Ratna Dewi,
Teti Estiasih, Erni Sofia Murtini
Kode Jurnal: jppertaniandd070026
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