EFEK PENGINJEKSIAN PRODUK INTRASELULER (ICP) DAN EKSTRASELULER (ECP) BAKTERI Pseudomonas sp. TERHADAP GAMBARAN DARAH IKAN NILA (Oreochromis niloticus)
ABSTRACT: This study
aimed to determine
the effect of
Pseudomonas ECP and
ICP injection incubated
on TSA and TSB
media with incubation
periad of 72
hours on the
nila tilapia (O.
niloticus) haematology.
Parameters observed were
hemoglobin, hematocrit, erythrocyte
total and of leukocyte
total. The ECP
or ICP ware injected into
intraperitial nila tilapia
as much as 0.2
ml/fish. Observations were
made on 6,
12, 24, 48,
72, 96, 120,
144, 168 hour
after injection. Extracellular
products were obtained by centifuging Pseudomonas suspension grown on TSA and TSB media
(incubation time 72 h + 30oC) using
a 10.000 g
centrifuge for 30
min at 4oC. Supernatant was filtered using filter
paper 0.45 μm. To get the ICP, supernatant
was centrifuged in room temperature. The results revealed that ICP
injection caused the decrease of haematology faster than ECP injection. This
indicates that the ICP Pseudomonas more virulent than ECP.
Keywords: haematology, ECP,
ICP, Oreochromis niloticus, Pseudomonas sp.
Penulis: Dosim, Esti Handayani
Hardi dan Agustina
Kode Jurnal: jpperikanandd130015
