EKSTRAKSI DAN KARAKTERISASI ENZIM POLIGALAKTURONASE ENDOJINUS PADA PULP BIJI KAKAO

ABSTRACT: Endogenous polygalacturonase (PG) enzymes in cocoa pulp have not been studied yet. The  research,  aimed  to  extract  and  characterize  endogenous  PG  enzymes  in  cocoa  pulp, was  carried  in  two  steps:  (i)  extraction  of  endogenous  PG  enzyme  and  (ii)  enzyme characterization  i.e.  enzyme  activity  assay  and  determination  of  enzymatic  kinetics  (Km and  Vmax),  molecular  weight  (MW)  by  electrophoresis,  and  determination  of  its  optimum temperature and pH. 
The  results  showed  that  the  characteristics  of  endogenous  PG  in  cocoa  pulp  were  as followed: the relative activity of 4,58± 0,06 Units/ml,  Vmax of 6,69 Units/ml, Km of 0,37 %, MW was 32,84 and 63,58 kDa, and the combination of temperature and pH optimum were 42,5oC and 4,6.
Key words: endogenous polygalacturonase (PG), cocoa pulp
Penulis: G.P. Ganda Putra, Harijono, Tri Susanto, Sri Kumalaningsih dan Aulanni’am
Kode Jurnal: jppertaniandd070005

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Jp Pertanian dd 2007