EKSTRAKSI DAN STABILITAS BETASIANIN DAUN DARAH (Alternanthera Alternanthera dentata) (KAJIAN PERBANDINGAN PELARUT AIR:ETANOL DAN SUHU EKSTRAKSI)
ABSTRACT: The aim of this
research was to know the temperature’s influence and the solvent comparison
(water:ethanol) on the betacyanin extract properties from blood leaf. The
research was also aimed to know the stability of betacyanin extract toward
temperature, pH, oxidant, and light of the best treatment. This research was conducted
in two factorial randomized block design. The first factor was extraction
temperature (4oC, 30oC and 60oC) and the second factor was water to ethanol
ratio (8:2, 5:5, and 2:8 (v/v). Each treatment combination was conducted in
triplication. The result of this research was analyzed using ANOVA, followed by
5% LSD test. If it had interaction, it would be continued by DMRT 5% test. The
best treatment was tested using De Garmo effectiveness index. The best
characteristic of extract was obtained from the water to ethanol ratio of 5:5
and extraction temperature of 30oC. Extract had physic-chemical properties as
follows: betacyanin content of 45,81 mg/100 g, yield of 81,05%, ethanol residue
of 0,099%, pH of 6,68, and color intensity L* of 24,4; a* of 4,7; and b* of
7,9. The stability of extract decreased as well as the increasing of heating
temperature, oxidant concentration (H2O2), and duration of lamp rays radiation,
and the extract stability increased at pH 5
Keywords: Betacyanin, solvent,
temperature, stability
Penuis: Ahmad Dhiaul Khuluq,
Simon Bambang Widjanarko, Erni Sofia Murtini
Kode Jurnal: jppertaniandd070024
