KAJIAN FERMENTASI BIOPLASTIK POLI -(3-HIDROKSIALKANOAT) (PHA) HIDROKSIALKANOAT) (PHA) OLEH Ralstonia eutropha Ralstonia eutropha MENGGUNAKAN SUMBER KARBON HIDROLISAT PATI SAGU PATI SAGU
ABSTRACT: Polyhydroxyalkanoates
(PHAs), microbial bioplastics, not only were similar in properties with those
made of petrochemical products, they were also completely biodegradable. At the
moment, the price of PHAs was still high. This research was conducted to
investigate the ability of Ralstonia eutropha to produce PHAs on a sago
starch-based substrate, one of the cheap and abundantly renewable resources in
Indonesia, and to characterize the PHAs produced. The microorganism was grown
on the hydrolyzed sago starch with an initial sugar concentration of 30 g/lIt
was found that the best value of the maximum specific growth rate (µmax)
was 0,188 h-1. Kinetic parameters at 96
hour-batch fermentation showed the respective final cell and PHA concentrations
of 4,41 g/L and 1,44 g/L. The yields of cell biomass (Yx/s), PHAs per gram of
cells (Yp/x), PHAs per gram of substrate (Yp/s) and the percentage of substrate
consumption (∆S/So) were, 0.15 g cell/g
sugar; 0.35 g PHA/g cells; 0.06 g PHA/g sugar and 99%, respectively. The PHAs produced were predominantly composed
of poly(3-hydroxybutyrate) (PHB) as the functional groups with a melting point
of 163,96 oC.
Keywords: hydrolyzed sago
starch, PHA, Ralstonia eutropha
Penulis: Nur Atifah, Khaswar
Syamsu, Ani Suryani
Kode Jurnal: jppertaniandd070022
