Mikroflora Saluran Pencernaan Ikan Gurame (Osphronemus gouramy Lacepede)
Abstract: The objectives of
this experiment was to find out micro flora that have proteolitic and
amylolytic activity in the intestine giant gouramy. Isolated in aerobe and
anaerobe condition eighth Tripticase Soy Broth (TSB, Merck) as a culture medium
added NaCl 1% with casein as an energy source for proteolitic and starch for
amylolitic, pH 7 and incubation temperature 29ºC. Isolate purified is done with
102 to 1010 dilution series, than transfer to Tripticase Soy Agar (TSA).
Identified pure isolate in a physiology and biochemistry manner. The intestine
giant gouramy obtained 12 isolate consist 4 isolate aerob proteolitic microbe
(Staphylococcus sp., Clostridium sp., Bacillus sp. and Moraxella sp.), 2
anaerob proteolitic microbe isolate (Nitrococcus sp. dan Aeromonas sp.), 3
aerob amylolitic microbe isolate (Mycobacterium sp. Lactobacillus sp. dan
Aeromonas sp.), and 3 anaerob amylolitic microbe isolate (Carnobacterium sp.,
Citrobacter sp. and Streptococcus sp.).
Key words: giant gouramy,
microflora, microbe proteolitic, amylolitic, aerob, anaerob
Penulis: Siti Aslamyah
Kode Jurnal: jpperikanandd090103
