Pemanfaatan Getah Papaya (Carica papaya L.) Kering Sebagai Sumber Enzim Proteolitik untuk Meningkatkan Derajat Pembuahan dan Derajat Penetasan Telur Ikan Mas (Cyprinus carpio L.)
Abstract: This experiment was
conducted to study on the effect of carp,s eggs washing by using crude papain solution in several
concentration on the fertilization rate
and the hatching rate. There were ten
treatments with tree replications each
as the eggs washing by using crude papain solution in 0 ppm,
0.01 5 second-EC0, 0.1 5
second-EC0, and 5 second-EC0, and tannin solution in 500
ppm that each was combined with
eggs rearing in methylene blue
solution in 0 ppm or 2 ppm. The experimental result showed that crude of papaya
latex of the
variety semangka paris
has proteolytic activity as much
as 1,068.67 U/g. The optimum
concentration of crude
papain solution for
five-second washing on the eggs was 22.8191 ppm. Washing of carp,s for five seconds by using crude papain solution
in 0.2281 ppm, 2.2819 ppm, and
22.8191 ppm (the 5 second-EC0) significantly increased
the fertilization rate from
48.994% on the
control (without crude
papain, tannin, or methylene
blue) to 49.057%, 49.632%, and
55.383%, and the
hatching rate from 51.314% on the
control to 51.424%, 52.404%,
and 62.194% respectively.
On the other hand the treatment significantly lowered the
percentage of S. megasperma-infected eggs from
10.403% on the control to
10.352%, 9.898%, and
5.359% respectively. Effectiveness of
the crude papain solution on the
fertilization rate and the hatching
rate was lower compared with that of
the 500 ppm of tannin’s.
Key words: dried
papaya latex, the proteolytic enzymes,
mucous layer, the 5-second EC0, the
percentage of S. megasperma-infected
eggs, the fertilization rate, the
hatching rate
Penulis: Ahmad Gufron Mustofa
Kode Jurnal: jpperikanandd090099
