PEMURNIAN PARSIAL ENZIM PROTEASE DARI GETAH TANAMAN BIDURI (Calotropis gigantea) MENGGUNAKAN AMMONIUM SULPHAT
ABSTRACT: The latex of
milkweed plant (Calotropis gigantea) is known to have proteolytic activities.
The research was conducted to evaluate the use of ammonium sulphate for partial
purification of such enzymes. The phosphate buffer extract of the latex was
first centrifuged to precipitate gum and non-proteinous matter. The supernatant
was then treated with ammonium sulphate to achieve the respective degree of
saturation, namely 35, 50, 65 and 80%. It showed that the use of ammonium
sulphate at 65% degree of saturation was the optimum condition, and produced a
partially purified enzyme with proteolytic activity which was not significantly
different from that obtained from the use of the same salt at 80% degree of
saturation. Nonetheless, the supernatant still showed a substantial degree of
proteolytic activity, indicating that the latex contained a various types of
proteases.
Key Word: Milkweed plant
(Calotropis gigantea), protease, partial purification
Penulis: Yuli Witono,
Aulanni'am, Achmad Subagio, Simon Bambang Widjanarko
Kode Jurnal: jppertaniandd060007
