PENGARUH PEMBERIAN KHITOSAN TERHADAP PROFIL LIPID TERHADAP SERUM DARAH TIKUS SPRAGUE DAWLEY
ABSTRACT: Chitosan shares
characteristic with dietary fiber which can interact with lipids and/or cholesterol in the intestinal lumen, consequently,
reduce their absorption. The effect of
chitosan concentration on the serum cholesterol levels (total cholesterol, HDL,
LDL cholesterol and trigliceride) and digesta profile of Sprague Dawley rats
were investigated. A two month old Sprague Dawley rats consisted of six groups
with seven animals of each group were adapted for one week by giving standard
diet (AIN-93). Then they were treated with hypercholesterol diet containing
approximately 200 mg/kg cholesterol for one week. At the end of this week, cholesterol level
was measured. The parameter for hypercholesterolemic rats was the raising level
of serum cholesterol as much as 130 mg/dl. The first three groups of rats were
treated with standard diet (AIN-93) with 0%, 2.5%, and 5% of chitosan. The second three groups were treated with
hypercholesterol diet with the same treatments as the first one. Blood was
drawn from eyes (reorbital plexus) every week for analyzing cholesterol levels
(total cholesterol, HDL, LDL cholesterol and triglicerides). At the end of the
treatment, seven rats were killed after one day fasting then liver and caecum
were collected for analyzing.Research showed that chitosan of 2.5% and 5% in
the diet did not alter body and liver weight of the group with standard diet (AIN-93)
treatment. Conversely, body wieght decreased and liver weight increased in the
hypercholesterol diet treatments. Chitosan also increased digesta weight and
cholesterol level either in standard or hypercholesterol diet treatments. Serum
profiles showed that chitosan treatment of 2.5% or 5% decreased total
cholesterol, LDL cholesterol and triglyceride but increased HDL cholesterol.
Keyword: lipid profile, blood
serum, chitosan, hypercholesterol
Penulis: Erryana Martati, Lily
Arsanti Lestari, Lily Arsanti Lestari
Kode Jurnal: jppertaniandd080020
