PRODUKSI BIOLAKTAT KERING KULTUR CAMPURAN Lactobacillus sp DAN Saccharomyces cereviceae
ABSTRACT: Recently,
fermentation process of cereal and legume–based products has been developed. In
the fermentation process, the aviability of starter cultures is needed. Starter
cultures that usually used to ferment cereal are Lactobacillus and yeasts.
Generally, the starter cultures are used in liquid form and it needs to be
periodically regenerated, that will lead to strain degeneration. To overcome
the problem and to make the starter cultures easy to use, it can be made in
dried form. In this research, we studied
to increase biomass cells of dried cultures that affected by some factors. The
aims of this research were to evaluate the suitable medium, growing methods,
substrate:culture ratio, and incubation time to get the highest biomass cells of dried starter cultures. Starter
culture in corn medium and the mixed culture method had the highest capability
for growing. Whereas, substrate to culture ratio (94:6) and incubation time of
24 hours showed the best viability of these microorganism, and the best dried
starter cultures with total BAL of 2.2X109 cfu/ml and total yeast of
3.0X108 cfu/ml. The best result on this
treatment was stored at room and refrigeration temperatures for 4 weeks and the
best stored proved on refrigeration which is can maintened viability of dried cultures.
Keywords: mixed culture, vacuum
drying, dried culture
Penulis: Widya Dwi Rukmi
Putri, Dewanti Widyaningsih, D. W Ningtyas
Kode Jurnal: jppertaniandd080018
