STUDI EFEKTIFITAS SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) UNTUK MENCEGAH PENYAKIT EDWARDSIELLOSIS PADA IKAN PATIN (PANGASIUS HYPOPTHALMUS)
Abstract: The aim of the
research was to discover the effectiveness of sambiloto(Andrographis paniculata
Nees) to prevent Edwardsiellosis infection caused byEdwardsiella tarda in
catfish (Pangasius hypopthalmus). Antibacterial activitytest has been carried
out from sambiloto solution on E. Tarda using disc diffusionmethod. The
concentration sambiloto solution use were 0, 1g/l, 2g/l, 3g/l and 4g/l.This
research show that sambiloto solution 5g/l forming the largest clear
zoneaverage 9,3 mm. The LD50 test of sambiloto solution given by immersion for
24 hat catfish can harm fish survival when given at doses above 7,30 g /
l.Treatment applied was the different dosage of sambiloto by immersion
tenminutes give for 30 days. Each fish was immersion with 1 g/l sambiloto (P1),
2 g/lsambiloto (P2) and 4 g/l sambiloto (P3). The fish was feed adlibitum
withcommercial fish feed pellets. Before 30 days treatment the fish
wereintramuscularly infected with E. Tarda with density of 10 7 cell/ml in a
dose of 0,1ml/fish.The best result was obtained in fish after challenged with
E.tarda show asignificantly between treatment in P3 (4g/l) (P ˂ 0.05), as the
survival rate was100 % and non-specific defence in the catfish show hematocrite
level is 22,67 %,total leucocytes is 31,70 thousand cell/mm3 and the percentage
of phagocytoticactivity at 55,5 %.
Keywords: Catfish,
Andrographis paniculata , antimicrobial, Edwardsiella tarda
Penulis: Isje Lukistyowati
Kode Jurnal: jpperikanandd120235