SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL
ABSTRACT: Microneme protein
(MIC) is one of proteins
that belongs to
excretory-secretory antigens
(ESAs) of Toxoplasma
gondii. Microneme 3
protein (MIC-3) is
the protein that plays
an important role
in the invasion
proccess during cell
infection as a
mediator attachment parasite to
the host cell.
The aim of
this research is
to clone mic3
(gene encoding for MIC-3) of T. gondii from local isolate using
recombinant DNA technology by cloning
mic3 in an
expression vector. Deoxyribonucleic acid
(DNA) from T.
gondii tachyzoites was amplified
by PuRe Taq
RTG-PCR Beads using
mic3 specific primers. Amplified DNA
was double digested
using EcoRV and
HindIII restriction endonucleases and then
purified using EZ-10
spin coloumn purification
kit. The mic3
DNA was ligated into
pET-32a(+) expression vector
and transformated into
Escherichia coli BL21.
The results showed that
recombinant mic3 gene
4.2 kDa has
been successfully performed
by cloning gene encoding
for MIC-3 protein
of T. gondii
local isolate into
pET-32a(+) and transformed to E.
coli BL21.
Keywords: Toxoplasma gondii,
cloning, MIC-3 protein
Penulis: Diana Indrasanti, Aris Haryanto dan Wayan T. Artama
Kode Jurnal: jppeternakandd110015
