Antioxidant Activity of Leave’s Extract and Isolate Ketepeng Kebo Cassia alata Linn (Leguminoceae)

Abstract: Phytochemistry  and  biological  activity  investigation  of  medical  plants was an important step in exploring of their potency. The objectives of this research were  to  determine  the  antioxidant  activity  of  the  extract  and  purified  isolate  of  C.alata  leave  and  also,  to  elucidate  of  the  molecular  structure  of  the  responsible molecule. The methods used were extraction, isolation and purification by means of chromatography, DPPH radical scavenging and spectroscopy. Result of the research provided  antioxidant  activity  was  (ED50)  28.50±1.86  µg/mL  for  the  extract  and 9.99±0.28  µM  for  the  purified  isolate.  Elucidation  toward  the  purified  isolate indicated  kaemferol  structure  as  the  most  active  antioxidant  compound.                   
Keywords: Antioxidant, Cassia alata, Leguminoceae, Kaemferol
Penulis: Masruri, Rurini R., Dasrul,  dan Aulann’iam
Kode Jurnal: jpkedokterandd070001

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