Ekspresi dan Aktivasi Reseptor Glukokortikoid serta Ekspresi Heat Shock Protein-90 pada Sel Limfosit Penderita Sindrom Nefrotik Idiopatik
ABSTRACT: Glucocorticoid
receptors (GR) have an important role in the resistance of steroid therapy.
Previous studies showed significant difference
activation and expression
of GR between
Steroid Resistant Nephrotic Syndrome (SRNS) and Steroid
Sensitive Nephrotic Syndrome (SSNS), however the other studies showed contrastive
result. Previous studies also revealed Hsp-90 had an important role in GR
activation. The aim of this study was to determine the differences expression
and activation of GR and also Hsp-90 expression in lymphocytes cytoplasm
and nucleus between
SRNS and SSNS
patients. A Cross-sectional study
was conducted to 40
children (20 SRNS
and 20 SSNS
patients). Double stainning
immunocytochemistry of lymphocytes was
performed. The GR
expression and activation
were determined by
counting amount of lymphocytes that express GR and Hsp-90
among 200 under light microscope with 1000x magnification. The differences of
activation and activation
of GR and
Hsp-90 in cytoplasm
and nucleus were
analyzed with independent t-test
and the correlation was analyzed with Pearson correlation. The study results
showed the GR expression and also the Hsp-90 in cytoplasm and nucleus in SRNS
was lower than SSNS group (p<0.05). There was no significant difference of
GR activation (p=0.347) and no correlation between GR and Hsp-90 in cytoplasm
(r=0.124).
Penulis: Khairiyadi, Krisni
Subandiyah, Loeki Enggar Fitri
Kode Jurnal: jpkedokterandd090146
