Ekspresi dan Aktivasi Sub Unit p65 NF-κB pada Sel Mononuklear Penderita Sindrom Nefrotik Resisten Steroid Anak
ABSTRACT: Resitance to steroid
therapy is a poor prognosis sign for nephrotic syndrome, but this mechanism is
still unclear. NF-κB has been reported to affect the glucocorticoid at the T
cell through the transrepression in the glucocorticoid receptor. Until know,
this study was still limited. The aim of this study was to show the difference expression
and activation of NF-κB p65 subunit between childhood SRNS and SSNS patient.
Cross sectional study was conducted
during January 2009
to December 2009
in the Saiful Anwar
General Hospital and Biomedic Laboratory Brawijaya University.
Expression and activation of NF-κB p65 subunit were determine by immunochemistry
staining by counting the amount of lymphocyte that express and activate NF-κB
p65 subunit among 200 lymphocytes
under light microscope
with 1000 magnification. Results
was analyzed by independent t
test with 95% CI (p<0,05)
and Pearson correlation.
The result of
this study revealed
no significant difference of expression of NF-κB p65 subunit between
SRNS and SSNS (p=0,153), but there was an increase activation of NF-κB p65
subunit in the SRNS patients (p=0,000). However, we did not find any correlation
between expression and activation of NF-κB p65 subunit (r=0.102, p=0.668). We
conclude that increasing of activation of NF-κB p65 subunit can cause
resistance to steroid therapy but it is not related with the expression of
NF-κB p65 subunit.
Penulis: Brigitta Ida RV
Corebima, Krisni Subandiyah, Loeki Enggar Fitri
Kode Jurnal: jpkedokterandd090148
