Ekspresi dan Aktivasi Sub Unit p65 NF-κB pada Sel Mononuklear Penderita Sindrom Nefrotik Resisten Steroid Anak

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ABSTRACT: Resitance to steroid therapy is a poor prognosis sign for nephrotic syndrome, but this mechanism is still unclear. NF-κB has been reported to affect the glucocorticoid at the T cell through the transrepression in the glucocorticoid receptor. Until know, this study was still limited. The aim of this study was to show the difference expression and activation of NF-κB p65 subunit between childhood SRNS and SSNS patient. Cross sectional study  was  conducted  during  January  2009  to  December  2009  in  the  Saiful Anwar  General  Hospital  and Biomedic Laboratory Brawijaya University. Expression and activation of NF-κB p65 subunit were determine by immunochemistry staining by counting the amount of lymphocyte that express and activate NF-κB p65 subunit among  200  lymphocytes  under  light  microscope  with  1000  magnification.  Results  was  analyzed  by independent  t  test  with  95%  CI  (p<0,05)  and  Pearson  correlation.  The  result  of  this  study  revealed  no significant difference of expression of NF-κB p65 subunit between SRNS and SSNS (p=0,153), but there was an increase activation of NF-κB p65 subunit in the SRNS patients (p=0,000). However, we did not find any correlation between expression and activation of NF-κB p65 subunit (r=0.102, p=0.668). We conclude that increasing of activation of NF-κB p65 subunit can cause resistance to steroid therapy but it is not related with the expression of NF-κB p65 subunit.
Keywords: nephrotic syndrome, steroid resistancy, expression and activation NF-κB p65 subunit
Penulis: Brigitta Ida RV Corebima, Krisni Subandiyah, Loeki Enggar Fitri
Kode Jurnal: jpkedokterandd090148

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Jp Kedokteran dd 2009