Peningkatan Ekspresi Interleukin (IL)-4 Berhubungan dengan Penurunan Bax dan Apoptosis Limfosit pada Bronkiolus dan Paru Mencit Asma
ABSTRACT: Decrease of
lymphocyte apoptosis is one factor that leads to chronic airway inflammation in
allergic asthma. Previous studies revealed the role of interleukin (IL)-4 in
preventing lymphocyte apoptosis; however there are few studies about the role
of lymphocyte-Bax and its relationship with IL-4 in asthma. The aim of the
study is to demonstrate IL-4 role in preventing lymphocyte apoptosis via
lymphocyte-Bax inactivation in bronchioli and lungs of asthmatic mice. It is a
randomized control group experimental study. The subject was Balb/c mice that categorized into
2 groups: asthma
and non-asthma. Asthma
group was sensitized
by ovalbumin intraperitoneally in
day 0 and 14, followed by inhalation every 2-3 days for 6 weeks. In week 8, all
of mice were terminated. The IL-4 and
lymphocyte-Bax expression was examined through immunohistochemistry method, whereas lymphocyte
apoptosis by TUNEL
method. Independent sample
t-test, Mann Whitney
U test, regression analysis,
and path analysis
was used in
statistical analysis with
confident interval 95%.
The bronchioli and lungs specimens were obtained from 18 mice (9 in each
group). Lymphocyte apoptosis was similar between 2 groups (p=0.116),
additionally lymphocyte-Bax decreased in asthmatic group (p=0.003). This
indicated low activity of lymphocyte apoptosis in asthmatic group. Conversely,
IL-4 expression increased 2in asthmatic group (p=0.00). There was moderate
negative correlation (r =0.29, p=0.011) between IL-4 and 2lymphocyte apoptosis.
There was also
strong negative correlation
(r =0.39, p=0.002) between
IL-4 and lymphocyte-Bax expression.
However, we did
not find any
correlation between lymphocyte-Bax and lymphocyte apoptosis (r=0.37, p=0.065).
Path analysis revealed the stronger direct relationship between IL-4 and
lymphocyte apoptosis rather than indirect relationship via lymphocyte-Bax
inactivation. We conclude that increases of IL-4 expressions inhibit lymphocyte
apoptosis; however, it is not due to the Bax-lymphocyte inactivation.
Penulis: Saptadi Yuliarto, HMS
Chandra Kusuma, Edi Widjajanto
Kode Jurnal: jpkedokterandd090143
