Peningkatan Jumlah Apoptosis Airway Smooth Muscle Berhubungan dengan Peningkatan +Jumlah Sel T CD8 pada Model Mencit Asma
ABSTRACT: Airway remodeling
is an important
thing in asthma.The
increase of airway
smooth muscle mass
is a prominent feature of
asthmatic airway remodeling. The importance role of airway smooth muscle cell
(ASM) + apoptosis in airway remodeling is unknown.T cell also had important
role in asthma, whereas CD4 T cell +role in airway remodeling has already
known, but how CD8 T cell role in airway
remodeling is still unclear. A randomized control group experimental study used
Balb/c mice, that categorized into 2 groups: asthma and control. Asthma group
was sensitized by ovalbumin intraperitoneally in day 0 and 14, followed by
inhalation +every 2-3 days for 6 weeks. In week 8, all of mice were
terminated. The expression of CD8 T cell lymphocyte was examined
through immunohistochemistry method,
whereas ASM apoptosis
by TUNEL method. Independent sample t-test and
spearman test was used in statistical analysis with confident interval 95%. The
bronchioli and lungs specimens were obtained from 18 mice (9 in each group).
Case goup had significant +increase in the amount of ASM apoptosis and
expression of CD8 T cell lymphocyte
(p=0.000; p=0.001). +There was also positif correlation between ASM apoptosis
and expression of CD8 T cell (r=0.37,
p=0.065). +We conclude that increasing
ASM apoptosis has a relationship with increasing CD8 T cell
Penulis: Dessy Setiawati, HMS
Chandra Kusuma, Kusworini
Kode Jurnal: jpkedokterandd090145
