BACTERIAL Cr (VI) REDUCTION AND ITS IMPACT IN BIOREMEDIATION
ABSTRACT: Chromium is
hazardous pollutant for ecosystem caused chromium especially in hexavalent form
is very toxic, has high solubility and mobility, teratogenicity, mutagenicity
and carcinogenicity to living system related with its oxiding power.
Remediation of soil contaminated of heavy metals was important caused soil as
medium for food producing. Conventional methods for heavy metals remediation
consist of physical and chemical process but these applications were costly and
less effective. One of the remediation technologies is the using living
organisms such as microorganisms, because they have ability to reduce Cr(VI)
into non toxic form, Cr(III). The aims of this research were to evaluate the
reduction activity of rhizobacterial isolate and to identify the isolate which
take a role in reducing chromium absorption by plant. The results showed that
Isolate 39 was able to grow on LB medium containing 200 ppm Cr(VI). Isolate 39
reduced Cr(VI) up to 15 ppm concentration level in minimal medium. Isolate 39
has ability to reduce Cr(VI) both at growing cells and resting cells conditions
up to 100% and 51% within 18 hours, respectively. Isolate 39 increased the phytostabilization
ability of chromium by Zea mays at 30 days after seeding 3.8 times compared
than control. Based on physiological characteristics and partial sequencing of
16S rRNA gene, Isolate 39 was identified as Agrobacterium sp.
Penulis: ALI PRAMONO, MMA
RETNO ROSARIASTUTI, N NGADIMAN, IRFAN D PRIJAMBADA
Kode Jurnal: jpkesmasdd130270
