ISOLASI OLIGOSAKARIDA MADU LOKAL DAN ANALISIS AKTIVITAS PREBIOTIKNYA
ABSTRACT: The objective of this
study was to isolate oligosaccharides from local honey of Pulau Sumbawa and
province of Kalimantan Timur and to analyze its prebiotic activity in vitro.
Oligosaccharides were isolated with activated charcoal adsorption and ethanol
treatment. Effectiveness of this method was evaluated by Thin Layer
Chromatography and colorimetry. The isolates were then being a sample to
prebiotic examination. Honey from Sumbawa forest has higher oligosaccharides
content than honey from Kalimantan. Qualitative and quantitative assays showed
that oligosaccharide isolation method was effective to concentrate the
oligosaccharide with high degree of polymerization but ineffective for mono-
and disaccharides removal. The highest percentages of hydrolysis of
oligosaccharide from Sumbawa and Kalimantan by mimic gastric juice were 1.78%
and 0.59 %. Whereas, the hydrolysis was higher with amylase, 11.87% for Sumbawa
and 21.03% for Kalimantan honey oligosaccharides. Prebiotic activity was 0.32,
and 0.09 for honey oligosaccharide from Sumbawa and Kalimantan respectively.
Honey oligosaccharide from Sumbawa presented higher prebiotic activity than
inulin as prebiotic reference (0.11). GC-MS chromatogram showed L. acidophilus
cultured in media added with Sumbawa honey oligosaccharide produced lactic acid
as the highest metabolite (48.01%).
Penulis: Umul Karimah, Yogi
Nur Anggowo, Syamsul Falah, Suryani
Kode Jurnal: jpkesmasdd110131
