Apoptosis Pneumosit Tipe I & II pada Syok Hipovolemik Perdarahan yang Diresusitasi dengan LSBT Studi Eksperimental pada Rattus Sprague Dawley
Abstract: Commonly used liquid
to correct the haemorrhagic hypovolemic shock is Ringer Lactate RL. However the
number of lung cell apoptosisin cases resuscitated with RL increased
three-fold. Liquid Substitution of Blood Transfusion (LSBT) is one of the
alternative fl uid thatcan be given in the pre hospital period with promising
advantages. The objective of this research was to verify whether apoptosis
ofpneumocyte type I and type II cells after resuscitation using LSBT is lower
than those resuscitated with RL. True Experimental Designon 30 Sprague Dawley
Rat were done. Hemorrhagic shock were induced by needle puncture on canthus
medialis and sustained for30 minutes. Rats were randomized into three groups,
10 subjects each, i.e. Group LSBT received LSBT 4ml/kg body weight as
theresuscitation fl uid, Group RL received Ringer Lactate 20 ml/kg body weight
and Group Bleeding which received no resuscitationfl uid. Lung tissue samples
were taken three hours after the completion of resuscitation for analysis of
Caspace 9, AIF, and MDA usingimmunohistochemical method. Tunnel method was used
to demonstrate apoptosis. Body weight, hemoglobin, natrium, lactate and
basedefi cit of the three groups of rats were homogenous. Both pneumocyte I and
pneumocyte II showed signifi cant increased activity ofthe caspace cascade as
measured by the expression caspace 9 and AIF after RL more than LSBT p = 0.000.
MDA expression amongpneumocte II showed no difference between LSBT and RL
groups p = 0.090. While signifi cant difference was found among pneumocyteI
between LSBT and RL, with higher MDA expression in the RL group p = 0.000.
Tunnel method to measure the apoptosis rate ofpneumocyte I showed signifi cant
difference in favor of LSBT p = 0.000 but not of pneumocyte II p = 0.090. LSBT
resuscitation showedsignifi cant benefi cial result on the lower apoptosis
rate, supported by both Caspace Cascade, AIF and MDA expression compared
toRinger Lactate. This new fact may open new opportunity to treat shocked
hemorrhagic patients with a new fl uid composition to achievebetter result with
less pulmonary complications.
Penulis: Kohar Hari Santoso, H.R.
Eddy Rahardjo, Harjanto
Kode Jurnal: jpbiologidd120052
