Bioaktivitas human Menopause Gonadotrophin (hMG) dan hMG Tergolikosilasi (hMGdG) dari Urin Perempuan Pascamenopause terhadap Terbentuknya Cleavage Embrio Sapi Secara In Vitro
Abstract: The aim of this
study was to produce hMG from the urine of post-menopausal women and to
evaluate the influence of glycan removal from hMG glycoprotein molecule
undergoing deglycocylation (hMGdG) in shortening the onset of cleavage embryo
of bovine in vitro. The experiments were performed on: 1) the identification of
hMG from the urine of postmenopausal women by confirming the glycoprotein
characteristic; 2) the examination of biochemical characteristic of hMG
deglycosylated using N-glycanase; 3) the influence of deglycosylated hMG on the
onset of embryo of bovine cleavage in vitro.Urine samples were collected from
30 postmenopausal women. The result of SDS-PAGE demonstrated protein bands with
molecular weight ranging from 19.4–107 kDa. Western Blot using anti-hMG
monoclonal antibody (Catalogue CSA-614 Stressgen Bioreagents) showed that
immunoreactivity at the band with molecular weight of 30 kDa which was a
glycoprotein. The band result of Western blot referred with MW 30 kDa was
glycoprotein. Glycoprotein is a protein molecule attached to carbohydrate
molecule by using Glycoprotein Carbohidrat Estimation Kit 23260. The
concentration rate of glycoprotein was 99860.00 μg/ml (PAS), protein was
66939.29 μg/ml (Biuret) and carbohydrate 32920.71 μg/mL (PAS). Concentration
ratio of glycoprotein, protein and carbohydrate from molecule hMG was3: 2: 1.
hMG from the urine of the 30 postmenopausal women was confirmed with the 30 kDa
molecular weight glycoprotein characteristic. The Chemical characteristic of
hMG undergoing enzymatic deglycosylation using N-glycanase reduced the
molecular weight into 26 kDa. The deglycocylated hMG reduced the onset of
cleavage embryo of bovine in vitro from 24 to 20 hours (p < 0.01).
Penulis: Herry Agoes Hermadi, L.
Mahaputra, Agus Abadi
Kode Jurnal: jpbiologidd110039
