Efek Jus Buah Tomat (Lycopersicum pyriforme) terhadap Pencegahan Fotoaging Kulit Akibat Iradiasi Sinar Ultraviolet-B
Abstract: Photoaging is caused
by a lack of collagen and skin elastine fibres due to external factors such as
solar UV which may give negative effects on skin, for examples, wrinkled,
pigmentation spotted, low elasticity and hard textures. The process of such an
early aging may be blocked or prevented by avoiding factors that may accelerate
it. However, so far no explanation has suggested that the effect of tomato
juice with a dosage of 11 g/kg Body Weigh may give effects on ROS activity,
AP-1 expression, and type-1 collagen expression in rat’s skin radiated with
UV-B with a dosage of 150 mJ/cm2. The objective of this study was to analyze
the effect of tomato juice on the prevention of collagen damage due to the
irradiation of UV-B light. The method of this research was used 24 has divided
into 4 groups consisting of 6 rat each. The control group were not irradiated
with UV-B or were not given tomato juice (P0). The experimental group were
given the following treatments: exposure to UV-B light of 150 mJ/cm2 (P1),
exposure to UV-B light of 150 mJ/cm2 + giving of tomato juice with the dosage
of 11 g/Kg/Body Weight (P2) exposure UV-B irradiation with the dosage of 150 mJ/cm2
+ combination application of lycopene, β-carotene, and vitamin C of which their
content is equal with that of tomato 11 g/kg body weight (P3). Treatments were
given to each group for 6 weeks. An experimental design using a cluster random
with 4 treatments and 6 replication was employed. AP-1 and type-1 collagen
expression, were measured with immunohistochemistry, and the MDA content with
NWLSS MDA Assay technique. The data were analysed using a variance analysis and
then followed with LSD Test. It can be concluded that the application of tomato
juice 11 g/kg body weight or of a combination of lycopene, β-carotene, and
vitamin C of which its content equals to 11 g/kg body weight may prevent to
increase the content of MDA (as indicator of ROS), AP-1 expression, but may
prevent to decrease the expression of type-1 collagen in the rat skin radiated
with UV-B 150 mJ/cm2. However, it also found that the application of a
combination of lycopene, β-carotene, and vitamin C of which its content equals
to 11 g/kg body weight may give no significant defference compared with the
application of tomato juice 11 g/kg body weight.
Penulis: Poncojari Wahyono, Soetjipto,
Harjanto, Suhariningsih
Kode Jurnal: jpbiologidd110056
