Efek Pemberian Glutamin terhadap Aktivitas Sucrase, Maltase, Lactase dan Ekspresi Spectrin, Clathrin dalam Perbaikan Mikrovili Ileum pada Tikus Malnutrisi
Abstract: Glutamine is the
most abundant amino acid in the blood and plays a key role of intestinal
response to local and systemic injuries suchas diarrhea and malnutrition. The
purpose of this study was to explain the effect of oral glutamine on sucrase,
maltase, lactase enzymeactivity and spectrin, clathrin expression also the
correlation with ERK-1 and STAT-3 signaling pathways in repairing
microvilliileum intestine in rat malnutrition. The method employed in this
study was experimental research, with control samples, measurableand reliable,
with the design of the study was post test only group design. showed that
twenty growing male rats (aged 12 week)were divided into 4 groups: control,
malnutrition, control+glutamine and malnutrition+glutamine. For the fi rst 15
days experiment,animals in the test groups received a karak diet (low calorie
diet of dried rice) for the next 15 days. Control and malnutrition
groupsreceived either glutamine supplement with the doses 500 mg/kg body
weight/day. On the 30th days, the animals were weighed andsacrifi ced of the
intestine was taken and prepared for enzymatic and expression examination.
Sucrase activity between malnutritionwith malnutrition+glutamine groups there
indicated signifi cance ileum (p = 0.04). Maltase activity when compared
malnutrition with malnutrition+glutamine groups there were signifi cant
differences in the ileum (p = 0.00). Lactase activity when compared
betweenmalnutrition with malnutrition + glutamine groups there were signifi
cant differences in ileum (p = 0.04). The expression of spectrinand clathrin
were evidence enlarge of enterocyte in the normal group after being given
glutamine compared to malnutrition groupafter being fed glutamine, spectrin and
clathrin expression appearance on ileum. Spectrin and clathrin molecular weight
protein in theileum with a molecular mass of 130-250 KDa. Relationship between
glutamine and meaningful pathway signaling proteins ERK-1 inthe ileum (p =
0.03) and also signifi cant association between glutamine and STAT-3 in ileum
(p = 0.00). The research proved that therewas an increase activity of sucrase,
maltase, lactase, also spectrin and clathrin which have expression differences
of each group. Thereare links between ERK-1 and STAT-3 signaling pathway in the
repairing microvilli of rat ileum intestinal damaged by malnutrition andimproved
after administration of oral glutamine.
Penulis: Roedi Irawan,
Subijanto MS, Suhartono Taat Putra, Soetjipto
Kode Jurnal: jpbiologidd120053
