INSERSI DAN DELESI INTRON SATU GEN RHODOPSIN (RDP1) NUKLEUS KELOMPOK BURUNG MEGAPODA (AVES MEGAPODIIDAE) INDONESIA
ABSTRACT: Insertion and
deletion are information that reflect evolutional potency of species or
population, that nowadays, are central topics in conservation genetics. This
research aims to reveal the proportion of insertion and deletion on RDP1of
species Megapodes in Indonesia. Whole blood DNA was extracted according to the
protocol of Qiamp DNA Blood Mini Kit (Qiagen), and used as template for PCR
(Polymerase Chain Reaction) which was conducted according the protocol of
Takara Ex TaqTM PCR Kit in a Perkin-Elmer Thermal Cycler 9600. PCR products
were purified using a Qiaquick PCR Purification Kit (Qiagen, USA). The
sequencing reaction for RDP1 was performed using BigDye v.3.1. Cycle Sequencing
Kit (Applied Biosystems,USA) followed the protocol of ABI Prism 3100-Avant
Genetic Analyzer (Applied Biosystems, USA). Alignment of RDP1 nucleotide
sequences of megapodes and that are obtained from international DNA data base
(DDBJ-DNA Data Bank of Japan) with accession number AF 394649 (RDP1) were
conducted by using Clustal-X. Characteristics of RDP1 were analyzed by
employing the DNA Sequence Polymorphism (DnaSP) version 3.51 and Program MEGA
version 4. The analysis result demonstrated there were insertion and deletion
on RDP1 of species of Megapodes in Indonesia, which resulting in a gap of 1-10
bp and was concentrated in nucleotide 407-568. Alignments between species of
Megapodes and two species of Galliformes
showed more gaps, which were concentrated on nucleotides 480-708.
Penulis: I Made Budiarsa
Kode Jurnal: jpbiologidd110103
