Karakterisasi dan Isolasi Protein Spesifik dari Material Excretory-Secretory (ES) Toxocara cati untuk Pengembangan Diagnostik Toxocariasis dengan Teknik ELISA
Abstract: The objective of
this study was to get the specific protein of T. cati that could be used as
biomarker in kit diagnostic to toxocariasis diagnosis through antibody test by
ELISA technique. This study was conducted by isolating and characterizis T.
cati protein from secretory-excretory (ES) material of L2 dormant (L2D) by pure
protein that was reactived with polyclonal antibody sera. For the first step,
ES protein of L2D was identified by SDS-PAGE with coumsie blue staining. The
second step, protein was transferred to nitrocellulose membrane by using
polyclonal antibody of anti-L2D T. cati that was visualized through goat-anti
rabbit conjugate and BCIP+NBT staining. The third step to decide the specific
protein fractions based on the molecule weight (MW), and then the specific
protein was isolated by elution technique. The fourth was to evaluate the
antigenicity, sensitivity and specificity of pure protein by IgG-ELISA as a
response against L2D T. cati immunization toward mice. The results of the study
showed that: T. cati have more protein with varied molecular weight ranging
from 8 to 200 kDa. The specific proteins of T. cati recognized by the anti-L2D
T. cati sera are the proteins of MW 168, 140, 120, 80, 70, 42, 32, 30, 24, 18,
14, 12 dan 10 kDa. The antigenicity of protein of T. cati was higher than that
of other worms (D_caninum and Ancylostoma spp.). The sensitivity of protein of
T. cati in mice with immunization was 100%, the specificity was 87.5% and the
false positive was 12.5%.
Penulis: Kusnoto, Sri Subekti,
I Ketut Sudiana, Soedarto
Kode Jurnal: jpbiologidd110044
Artikel Terkait :
Jp Biologi dd 2011
- Kondisi Parameter Biologi Plankton dan Ikan di Perairan Danau Sentani
- Profil Mamalia Kecil Gunung Slamet Jawa Tengah
- Pemberian pelet formula pada landak Jawa (Hystrix javanica F. Cuvier, 1823) : pengaruhnya terhadap konsumsi, konversi pakan, dan kecernaan pada kondisi pra budidaya
- Analisis Fekunditas dan Diameter Telur Kerang Darah (Anadara antiquata) di Perairan Pulau Auki, Kepulauan Padaido, Biak, Papua
- Serapan Karbondioksida (CO2) Jenis-Jenis Pohon di Taman Buah "Mekar Sari" Bogor, Kaitannya dengan Potensi Mitigasi Gas Rumah Kaca
- Dimorfisme Seksual, Reproduksi dan Mangsa Kadal Ekor Panjang Takydromus sexlineatus Daudin, 1802 (Lacertilia :Lacertidae)
- Kajian Hubungan Antara Fitoplankton dengan Kecepatan Arus Air Akibat Operasi Waduk Jatiluhur
- Eritrosit dan Hemoglobin pada Kelelawar Gua di Kawasan Karst Gombong, Kebumen, Jawa Tengah
- Sintesis Alkil N-asetilglukosamina (Alkil-GlcNAc) dengan Enzim N-asetilheksosaminidase yang diisolasi dari Aspergillus sp. 501
- Bentuk Sel Epidermis, Tipe dan Indeks Stomata 5 Genotipe Kedelai pada Tingkat Naungan Berbeda
- Tingkat Eksploitasi Ikan Endemik Bonti-bonti (Paratherina striata) di Danau Towuti
- Morfologi Larva dan Pola Infeksi Falcaustra kutcheri Bursey et.al., 2000 (Nematoda : Cosmocercoidea: Kathalaniidae) Pada Leucocephalon yuwonoi (McCord et.al., 1995) Di Sulawesi Tengah, Indonesia
- Bird Community Structure in Karimunjawa Islands, Central Jawa
- Genetic Variation of Agathis loranthifolia Salisb. in West Jawa Assessed by RAPD
- Forest Condition Analysis Based on Forest Canopy ClosureWith Remote Sensing Approach
- Phylogenetic relationships within Cockatoos (Aves: Psittaciformes) Based on DNA Sequences of The Seventh intron of Nuclear β-fibrinogen gene
- Perbandingan Hasil Belajar Biologi Materi Sistem Saraf Dengan Menerapkan Model Pembelajaran Kooperatif Tipe Grup investigasi dan Model Pembelajaran Langsung Pada Siswa Kelas IX IPA SMA Negeri 1 Sungguminasa
- Pengaruh Pendekatan Inquiry dan Discovery Terhadap Hasil Belajar Biologi Siswa Kelas XI SMA Negeri 1 Walenrang
- Indeks Keanekaragaman Makrozoobentos Sebagai Bioindikator Tingkat Pencemaran Di Muara Sungai Jeneberang
- Keanekaragaman Jamur Basidiomycota Di kawasan Gunung Bawakaraeng (Studi Kasus: Kawasan Sekitar Desa Lembanna Kecamatan Tinggi Moncong Kabupaten Gowa)
- Pengaruh Penerapan Model Pembelajaran Kooperatif Tipe Giving Question dan Getting Answer Pada Konsep Sistem Gerak Terhadap Hasil Belajar Siswa SMAN 4 Bantimurung
- Analisis Kadar Etanol Hasil Fermentasi Ragi Roti Pada Tepung umbi Gadung (Dioscorea hispida Dennst) terhadap kadar etanol
- Implementasi Soal-Soal Berstruktur Berbasis Information and Communication Technology (ICT) untuk Meningkatkan Aktivitas dan Hasil Belajar Biologi Siswa Kelas XI IPA1 SMA Negeri 1 Tellu Limpoe Kabupaten Sidenreng Rappang
- Pengaruh Cara Pengolahan Umbi Tire (Amorphophallus sp.) terhadap Kadar Kalsium Oksalat
- Potensi Bakteri Zoogloea Sp sebagai Bakteri Pembentuk Bioflok pada Sistem Pertambakan