Mekanisme dan Aktivitas Antimalaria dari Senyawa Flavonoid yang Diisolasi dari Cempedak (Artocarpus Champeden)
Abstract: This study was aimed
to monitor Flavonoid Compound activity and mechanism as antimalaria that
isolated from A. Champeden. Two new prenylated flavones,
5,7,2′,5′-tetrahydroxy-4′-methoxi-3-prenylflavone and
5,7,2′,4′-tetrahydroxy-8-prenylfuranodihydrobenzoxanthone, named after artocarpones
A and B respectively, have been isolated from the stembark of Artocarpus
champeden, together with six known isoprenylated flavonoids: artonin A,
cycloheterophyllin, heterophyllin, artoindonesianin R, heteroflavanone C,
artoindonesianin A-2, and one flavanone artoindonesianin E. Their structures
were determined on the basis of spectroscopic analysis. The isolated compounds
were all tested for their antimalarial activity against the growth of
Plasmodium falciparum 3D7 in vitro and the results indicated that all but one
compound, artoindonesianin E (IC50 = 75.76 μM), inhibited the parasite growth
significantly. The highest antimalarial activity was shown by heteroflavanone C
(IC50 = 0.001 μM). An attempt to determine its mechanism of action by using sorbitol-induced
hemolysis inhibitory assay revealed that artoindonesianin R, artocarpone A and
artoindonesianin A-2 showed hemolysis inhibition over 60% and, therefore,
identified as inhibitor of the NPP of the infected erythrocyte. The results
indicated that flavonoid compounds from this A. champeden exhibited potent
antimalarial activity through either inhibition of the parasite inducing new
permeation pathway or other yet unidentified mechanism. Therefore, the
compounds are potential to be developed as new antimalarial drugs, through
phytopharmaceutical product of its stembark or synthesized products of the
compounds.
Penulis: A ty Widyawaruyanti,
Noer Cholis Zaini, Syafruddin
Kode Jurnal: jpbiologidd110045
