Mekanisme Pneumonitis Hipersensitif (Hp) Akibat Pajanan Debu Penggilingan Padi pada Mencit (Mus musculus) BALB/C
Abstract: The purpose of this
research was to analyze the immune response mechanism of Hypersensitivity
Pneumonitis (HP) as a result fromthe exposure of dust from paddy milling on
mice (Mus musculus)BALB/C. The research done was a laboratory experimental
researchwith mice (Mus musculus) as experimental animal. The research design
used was the post only control group design using mice (Musmusculus) Balb/c as
experimental animal. Mice (Mus musculus) BALB/C were exposed to dust from paddy
milling for four (4) hours/dayand it was done for thirty (30) days with the
exposed concentrations respectively were 0.50 mg/m3, 0.75 mg/m3, 1.00 mg/m3.The
researchvariables were free variable, dependent variable, and control variable.
Independent variable was dust from paddy milling, dependentvariables were
Hypersensitivity Pneumonitis (HP), IgE, IL-4, CD8, IFN-γ, infl ammatory cells,
and histopathological picture of micelung, while control variables were strain,
body weight and age of mice (Mus musculus) BALB/C. The research result showed
that therewas an increased of IgE, yet statistically there was no signifi cant
difference; there was an increase on IL-4, CD-8, IFN-γ, infl ammatorycells and
lung histopathology and statistically there was a signifi cant difference
between the study and control on mice BALB/C. Theconclusion of the research was
that the immune response mechanism of Hypersensitivity Pneumonitis (HP) as a
result from the exposureof dust from paddy milling on mice (Mus musculus)
BALB/C. It could be concluded that dust from paddy milling inhaled
repeatedlypassed into the alveoli and then it was caught by alveolar
macrophages which then generated the increase of IL-4 and CD-8. Afterthat, IL-4
generated the increase of IgE which afterward attracted mast cells while CD8
expressed IFN-γ which then activated alveolarmacrophages and attracted a number
of neutrophil and mast cell which subsequently induced infl ammation. The infl
ammation occurredwould develop into other tissue damage and led to
Hypersensitivity Pneumonitis (HP). It was suggested that: it be better to
repeat withlonger exposure time so that the damage in lung be seen more clearly
especially the occurrence of granuloma in lung.
Penulis: Isa Ma’rufi, Kuntoro,
Soedjajadi Keman, JF. Palilingan
Kode Jurnal: jpbiologidd120050
