Mikrodelesi Gen RBM dan DAZ pada Pria Pasangan Infertil dalam Masyarakat yang Melakukan Kawin Kerabat
Abstract: The existing
phenotypic variation depends on various sites of deletion, the extent of
deletion, ethnicity, geographical location, and population typology. RBM (RNA
Binding Motive) and DAZ (Deleted in Azoospermia) are genes located at
chromosome Y (Yq11). The deletion of these genes is related to subnormal
spermatogenesis. This study was carried out to male partner of infertile couple
who underwent inbreeding in a closed population that lived in a hilly area 40
meters above sea level. This study observed dynamic population, the prevalence
of infertility, determination of RBM and DAZ genes deletion in male partner of
infertile couple, the nucleotide sequence of both genes, the detection of urinary
Chlamydia trachomatis using PCR, and blood FSH, LH, and testosteron levels. The
objective of the study was to find out the prevalence of RBM and DAZ genes in
male partner of infertile couple in population with inbreeding and to identify
possibility of infertility due to factors other than deletion, such as
Chlamydia trachomatis infection at reproductive tract and hormonal factors,
i.e., FSH, LH and testosteron.The result showed that the prevalence of
infertility among population was 19%, the prevalence of RBM and DAZ genes
deletion was 50% of male infertile couples, and the prevalence of deletion of
only one, RBM or DAZ, or both RBM and DAZ, was 65%. The factors of Chlamydia
trachomatis infection and hormones of FSH, LH, and testosteron showed no significant
effect as the cause of infertility. The rate of the prevalence of both genes
deletion was relatively high compared to that found in similar studies on the
population without inbreeding, although the subjects, the azoospermic group
(18%), had been accurately selected.Less developed and relatively small number
of population (309), closed type (inbreeding), lower fertility rate, and the
birth of male infants in larger number became adverse factors from the aspect
of population growth. The deletion of RBM and DAZ genes inherited in male
spring, inbreeding, and closed population may increase the ratio of deletion
prevalence, so that the male infertility also increase. Inconclusion, the
prevalence of RBM and DAZ genes deletion is relatively high in male partner of
infertile couple who undergoes inbreeding.
Penulis: Hudi Winarso, P.G.
Konthen, F.M. Judajana
Kode Jurnal: jpbiologidd110042
