Pengaruh Escherichia coli dan Lekosit terhadap kualitas spermatozoa manusia secara in vitro
Abstract: Male genital tract
infection has been recognized as one of the causes of infertility. Indicators
of the occurrence of genital tract infection is the presence of bacteria during
semen culture (bacteriospermia) and the finding of leucocyte of more than 1 million/ml
semen (leucotytospermia). E. coli is the most common cause of prostatitis and
epididymitis. The objective of this study was to explain the effect of E. coli
and leucocyte on spermatozoa quality in vitro. This study comprised three
experiments. In experiment 1 sperm was incubated with E. coli, in experiment 2
with lymphocyte, and experiment 3 with granulocyte. In those experiments, sperm
motility and vitality. Spermatozoa were obtained from donor with normal
spermatozoa according to WHO (1999). E. coli was obtained by culturing the
semen of infertile males. Lymphocyte and granulocyte were obtained from donors’
blood. Sperm preparation was made using Percoll gradient column method.
Lymphocyte isolation was performed using Histopaque 1077, and granulocyte
isolation used Histopaque 1077 and 1119. Sperm motility and vitality was
observed by means of conventional method with microscope. The result of this
study showed that in vitro E. coli had effect on the motility (p = 0.000) and
vitality(p = 0.000) of sperm. Lymphocyte had effect on the motility (p = 0.034)
and vitality (p = 0.041). Granulocyte had effect on motility (p = 0.000) and
vitality (p = 0.017). In conclusion, E. coli has effect on the reduction of
sperm quality through E. coli adhesion to the sperm. In leukocyte, granulocyte
has higher effect in the reduction of sperm quality. Sperm quality reduction by
granulocyte undergoes through the mechanism of ROS production.
Penulis: S ukarjati, Doddy M
Soebadi, Aucky Hinting, Sudjarwo
Kode Jurnal: jpbiologidd110053
