PENGGUNAAN DARAH KADARLUARSA SEBAGAI MEDIA ISOLASI DAN IDENTIFIKASI STREPTOCOCCUS FAECALIS
ABSTRACT: Efforts to find
microbes in microbiological examination of material are carried out by means of
isolation and identification of microorganisms from the examination material.
Isolation was carried out using culture medium. Use a medium for the isolation
and identification are closely related with one of Koch's postulates, that is causing
the infection must be cultured in the laboratory. Research on the use of
expired blood as a medium of isolation and identification of Streptococcus
pyogenes has been performed. The aim study is to determine the viability of the
colony and the size of the zone of hemolysis of Streptococcus pyogenes in the
blood as a medium expired. Materials use is expired blood 1, 2, 3 and 4 weeks.
Isolation procedures and identification of Streptococcus pyogenes include Gram
stain, type of hemolysis, catalase test, and test basitrasin. The results
showed the blood expired before four weeks show the number of colonies was
almost the same viability with sheep blood agar medium and large blood
hemolysis zone are also nearly equal to expire so that the blood of sheep prior
to 4 weeks. Thus the blood is exceeded before the 4 weeks can be used as media
for the isolation and identification of Streptococcus pyogenes
Penulis: Mudatsir
Kode Jurnal: jpbiologidd100078
