Peningkatan L-type Calcium Channel, Kalsium Intraseluler, Protease Activating Receptor-1 dan Heat Shock Protein-27 pada Uterus Kelinci New Zealand yang Dilakukan Penjahitan Modifikasi Surabaya (Kajian Biomolekuler pada penanganan Perdarahan Pascapersalinan karena Atonia Uteri)
Abstract: Uterine atony as the
common cause of PPH, which causing high maternal mortality especially in under
developed and developingcountries, can indeed be largely rescued by compression
with conservative surgical procedure by performing Surabaya suture
compressionmodifi cation (SSCM). The purpose of this study was to analyze the
increasing expression of L-type calcium channel, PAR-1 and HSP-27and density of
intracellular calcium on animal model of aterm pregnant New Zealand rabbit uterus.
True experimental single blindedstudy was done in Airlangga University
Veterinary Faculty and Brawijaya University Physiology Department from
March–August2011. Twenty two samples were obtained over this study which
applied on aterm pregnant rabbit uterus as animal model. Animal modelconsisted
of rabbit uterus in which practiced on by SSCM in 11 samples after terminating
pregnancy and removing pup and placentaas well, and the remainder of another 11
samples without SSCM as the control. The assessment of L-type calcium channel,
PAR-1 andHSP-27 expression and density of intracellular calcium on myometrium
sample was done respectively by immunohistochemistryand fl uoro-2 double
staining method at ½, 1, 2, and 24 hours intervals. For all times intervals the
increasing expression of L-typecalcium channel was observed signifi cantly in
SSCM compared to control (p = 0.001; p < 0.0001; p = 0.011 and p <
0.0001), alsofor intracellular calcium density (p = 0.022; p = 0.001; p = 0.005
and p = 0.002). The study showed PAR-1 and HSP-27 expressionincreasing sharply
that statistically analysis indicated both all of p similarly < 0.0001
against control. SSCM suggested stronglyincreasing some component (all
variable) that absolutely required to myometrial contraction.
Keyword: PPH, Surabaya suture
compression modifi cation, L-type calcium channel, PAR-1, HSP-27 and
intracellular calcium
Penulis: Agus Sulistyono, Agus
Abadi, Endang Joewarini, F.M. Judajana
Kode Jurnal: jpbiologidd120054
