Peningkatan L-type Calcium Channel, Kalsium Intraseluler, Protease Activating Receptor-1 dan Heat Shock Protein-27 pada Uterus Kelinci New Zealand yang Dilakukan Penjahitan Modifikasi Surabaya (Kajian Biomolekuler pada penanganan Perdarahan Pascapersalinan karena Atonia Uteri)

Abstract: Uterine atony as the common cause of PPH, which causing high maternal mortality especially in under developed and developingcountries, can indeed be largely rescued by compression with conservative surgical procedure by performing Surabaya suture compressionmodifi cation (SSCM). The purpose of this study was to analyze the increasing expression of L-type calcium channel, PAR-1 and HSP-27and density of intracellular calcium on animal model of aterm pregnant New Zealand rabbit uterus. True experimental single blindedstudy was done in Airlangga University Veterinary Faculty and Brawijaya University Physiology Department from March–August2011. Twenty two samples were obtained over this study which applied on aterm pregnant rabbit uterus as animal model. Animal modelconsisted of rabbit uterus in which practiced on by SSCM in 11 samples after terminating pregnancy and removing pup and placentaas well, and the remainder of another 11 samples without SSCM as the control. The assessment of L-type calcium channel, PAR-1 andHSP-27 expression and density of intracellular calcium on myometrium sample was done respectively by immunohistochemistryand fl uoro-2 double staining method at ½, 1, 2, and 24 hours intervals. For all times intervals the increasing expression of L-typecalcium channel was observed signifi cantly in SSCM compared to control (p = 0.001; p < 0.0001; p = 0.011 and p < 0.0001), alsofor intracellular calcium density (p = 0.022; p = 0.001; p = 0.005 and p = 0.002). The study showed PAR-1 and HSP-27 expressionincreasing sharply that statistically analysis indicated both all of p similarly < 0.0001 against control. SSCM suggested stronglyincreasing some component (all variable) that absolutely required to myometrial contraction.

Keyword: PPH, Surabaya suture compression modifi cation, L-type calcium channel, PAR-1, HSP-27 and intracellular calcium

Penulis: Agus Sulistyono, Agus Abadi, Endang Joewarini, F.M. Judajana

Kode Jurnal: jpbiologidd120054

Peningkatan L-type Calcium Channel, Kalsium Intraseluler, Protease Activating Receptor-1 dan Heat Shock Protein-27 pada Uterus Kelinci New Zealand yang Dilakukan Penjahitan Modifikasi Surabaya (Kajian Biomolekuler pada penanganan Perdarahan Pascapersalinan karena Atonia Uteri)

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