Peran TGF-b1 sebagai Regulator Switching Isotype sekresi sIgA Saliva
Abstract: IgA Isotype
switching requires the induction of TGF-β1 to regulate transcriptional
activity. Disturbances in the differentiation of B cells can occur because of
an increasing or decreasing in expression of TGF-β1. This difference causes the
differentiation of B cells disrupted because TGF-β1 play a role in DNA
re-arrangement for isotype switching in B cells, causing changes in
membrane-bound antibody IgM became membrand-bound antibody IgA. This Study was
aimed to identity and coralabe between sIgA with TGF beta. This research was
conducted through the 30 samples from the case group with sIgA levels> 300
ng/ml and 30 samples from the control group with sIgA levels < 300 ng/ ml.
Gingival tissues obtained during the extraction of decidous teeth. Gingival
tissue was inserted into a solution of 10% buffered formalin. Preparation of
histological preparations followed by immunohistochemical staining procedure using
monoclonal antibody anti-TGF-β1. This result showed that statistical tests of
TGF-β1 in the case group contained an average of 26% of the entire field of
view of the lamina propria. Average in the control group by 57% of the entire
field of view of the lamina propria. Data on the number of TGF-β1 in the case
and control groups are normally distributed. The results of the analysis by t
test found significant differences in the mean number of TGF-β1 in the case and
control groups (p = 0.000). There is a strong correlation between TGF-β1 and
sIgA. Decreased of TGF-β1 expression associated with decreased activation in
the sIgA isotype switching, and vice versa.
Penulis: Pratiwi Soesilawati, Harianto
Notopuro, Istiati Soehardjo, Afaf Baktir
Kode Jurnal: jpbiologidd110052
