Potensi Blue Light Emitting Diode (LED) untuk Fotoinaktivasi Bakteri Staphylococcus aureus dengan Porfirin Endogen
Abstract: Photodynamic
Inactivation (PDI) represents a method for bacteria inactivation by using
combination of endogenous porphyrin and light photon. The purpose of this study
were to determine the instrument performance of LED light source that had been
assembled and to recognize the potency of LED irradiating energy dose for
photoinactivation of Staphylococcus aureus bacteria ATCC 6538P with endogenous
porphyrin. Performance measurement of LED instrument showed that blue LED had
emission peak of 430 nm and red 629 nm with 65 nm and 30 nm bandwidth. The
analysis of LED irradiating energy dose by anova indicated that interaction of
power and irradiating time of blue LED 430 nm decreased the percentage of
Staphylococcus aureus colonies. With optimal irradiating of 75 mW/cm2 power
density and 30 minute time of irradiating (energy density 135 J/cm2), it
yielded the percentage decrease of Staphylococcus aureus colonies as many as
75%. Hence, the blue LED instrument has a high potential for photoinactivation
to Staphylococcus aureus bacteria.
Penulis: Suryani Dyah Astuti, Ni’matuzahroh,
Muhammad Zainuddin, Suhariningsih
Kode Jurnal: jpbiologidd110055
