POTENSI MIKROORGANISME SEBAGAI AGEN BIOREMIDIASI DALAM MENURUNKAN KADAR Cr (vi) DALAM LIMBAH CAIR TEKSTIL HASIL PEWARNAAN
ABSTRACT: Hexavalent chrom
(Cr(VI)) is recognized as toxic heavy metal, produced from textile printing
manufacture. Negatif effect of Cr(VI)
shuold be reduced towards biotic environtment, so it is important to reduce
Cr(VI) become Cr(III) which little hazard. Pseudomonas aeruginosa, Pseudomonas
putida, Klebsiella pneumonia, Pantoea sp and Saccharomyces cerevisieae. They
are including as resistent Cr(VI) microorganism and they have ability to reduce
Cr(VI). The aim of the research is to know the ability of microorganism in
reducing Cr(VI) and knowing protein band pattern between Cr(VI) resistent
microorganism and microorganism which is inoculated on LB broth without
Cr(VI). Electrophoresis SDS-PAGE is the
methode which is used to indentified protein expression. While, for knowing
concentration Cr(VI) in liquid medium is used by 1,5 difenilkarbazid methode.
The quantitative data is obtained being analyzed by ANAVA two factorial
continued with Duncan test at 1% level test. The qualitative data include
protein expression analyzed by Relative mobility (Rf) and for knowing molecular
weight of protein is used protein marker. The qualitative data had been
analyzed by descriptive qualitative methode.
The result of the research shows that
in Cr(VI) concentration after being treat by the microorganisms at the
initial concentration 0, 5ppm, 1 ppm, 5 ppm and 10 ppm and compared the reduce Cr(VI) capability from the average
procentage data each microorganism are: P. putida (65%) > S. cerevisieae
(64,45%) > P. aeruginosa (60,73%) > Pantoea sp (50,22%) > K. pneumonia
(47,82%) > without microorganism (34,25%). The adding microorganisms have
influence toward reduction of Cr(VI).
Penulis: Umi Fatmawati, Sajidan,
Suranto
Kode Jurnal: jpbiologidd100120
