PRODUKSI DAN PENGUKURAN AKTIVITAS PROTEASE DARI ISOLAT BAKTERI BKL-1 DAN BKU-31
ABSTRACT: BKL-1 and BKU-31
bacteria isolates were collected from digestive tract of kerapu lumpur
(Epinephelus tauvina) and identified as Serratia marcescens biogp 1 dan
Serratia marcescens respectively. BKL-1 crude protease were obtained after 27
hours incubation period in Nutrient Broth (NB) medium added with peptone 0.5%,
NaCl 0.5%, lactose 0.1%, and CaCl2 0.1%. BKU-31 crude proteases were obtained
in NB medium added with 0.1% skimmed milk (NBS) for 18 hours incubation period.
The highest activity of proteases of S. marcescens biogp 1 BKL-1 reached after
27 hours of incubation, namely 0.002 U/ml with protein concentration 0.064
mg/ml. The proteases of S. marcescens BKU-31, on the other hand, reached their
highest activity (0.064 U/ml) at 24 hours of incubation with protein
concentration 0.056 mg/ml.The optimum temperature crude BKL-1 proteases were
30°C, whereas BKU-31 proteasess had the optimum temperature of 50 ºC.
Penulis: Wiwit Artika
Kode Jurnal: jpbiologidd100069
