Regenerasi pada Massive Bone Defect dengan Bovine Hydroxyapatite sebagai Scaffold Mesenchymal Stem Cell
Abstract: Massive bone defect
caused by various diseases and disorders, until now is still a major problem in
the field of Orthopedics and Traumatology, because if not handled properly can
cause permanent disability in patients. Massive bone defect reconstruction need
big graft size, and the ideal graft must have characteristics of
osteoconductive, osteoinductive, and osteogenesis.This study aimed to prove
that there will be bone regeneration process take place in massive bone defect
which being treated with bovine hydroxyapatite as scaffold of mesenchymal stem
cells.This was a post test only control group design study. Experimental unit
was male New Zealand white rabbit, which in this research will be underwent
cutting of ulna osteoperiosteal as critical sized bone defect, then
reconstruction was performed using a bovine hydroxyapatite (control group) and
composite of bovine hydroxyapatite with mesenchymal stem cells. After 8 weeks,
the reconstructed ulna has been were taken and being analyzed. Analysis results
from control and treatment group were: no difference on radiological
examination (p = 0.133), histopathological examination showed difference in total
matrix formed (p = 0.00), difference in union distal and proximal (p = 0.000
and p = 0.015), matrix in graft also showed to be different (p = 0.000),
difference in bone matrix (p = 0.010). Immunohistochemistry examination showed
difference in collagen type I and osteocalcin (p = 0.000 and p = 0.000).
Correlation was found between collagen type I bone matrix (p = 0.039 dan p =
0.049). Multivariate analysis showed correlation between osteoblast product and
massive bone defect regeneration (p = 0.797). The conclusion of this research
was regeneration occur in the reconstruction of massive bone defects using a
composite graft of bovine hydroxyapatite with mesenchymal stem cells.
Penulis: Ferdiansyah, Djoko
Rushadi, Fedik Abdul Rantam, Aulani'am
Kode Jurnal: jpbiologidd110057
