PEMBENTUKAN KALUS TANAMAN ROSELLA (Hibiscus sabdariffa) PADA PEMBERIAN NAFTALEN ACETYL ACID (NAA) DAN BENZYL AMINO PURIN (BAP) SEBAGAI SUMBER BELAJAR KONSEP BIOTEKNOLOGI
Abstract: Has
conducted research for a hand out submateri biotechnology high school students
based on plant tissue culture research Roselle (Hibiscus sabdariffa). The study
includes two phases, namely: (1) Experiments to produce callus tissue culture
experiments using completely randomized design (CRD) factorial. The first
factor is the level of NAA treatment that is 0, 1,1.5, 2 and 3 mg/l. The second
factor is the level of BAP treatment with 0, 0.5, 1, and 1.5 mg/l. Each
treatment was repeated 3 times; (2) Preparation of handouts from the research
as source of learning the ADDIE model
(Analysis, Design, Development, Implementation, and Evaluation).Parameters
measured were the percentage of growing explants, while appearing callus and
callus texture. Analysis of data growing percentage of explants with advanced ANOVA
and DMRT test at 5% level, while the current and emerging callus texture descriptively.
In a growing percentage of explants parameter combination treatment showed a percentage
of 100 % are in treatment A 0 B 1,5 , A 1 B 0,5 - A 3 B 1,5 . For the current
parameters appear callus treatment best combination found in treatment A 3 B
1,5 with a mean time of 2 HSK appear callus (days after culture) and for the
texture parameters of combination treatment showed callus crumb texture and
white color found in treatment A 2 B 0,5 - A 3 B 1,5 , the research on callus
growth can be used as a learning resource in the form of handouts for high school
level students
Penulis: Imam Mahadi, Sri
Wulandari, Addarwida Omar
Kode Jurnal: jpbiologidd140084
