PENGARUH WAKTU PEMBENTUKAN DAN KESTABILAN HIDRINDANTIN SERTA KONSENTRASI NINHIDRIN PADA PEMBUATAN TES KIT SIANIDA
Abstract: Cyanide is a
compound which has a potential to harm human health even causes to death. The
existing methods for cyanide analysis can’t be done by all societies because
they require certain expertise, expensive, and time consuming. Alternative method in the form of a test kit
has been developed for cyanide determination by the formation of a blue
hydrindantin in the presence of ninhydrin. The performance of test kit were
optimized towards the time of formation and stability hydrindantin as well as
the concentration of ninhydrin. Optimization was done by measuring the
absorbance using Spectronic-20 at maximum wavelength of 590 nm under alkaline
condition (pH 12). The blue hydrindantin was formed spontaneously and stable
for 0-30 minutes. After 30 minutes, color of hydrindantin was faded. Thus, 5
minutes was chosen as optimum time suits to measure cyanide using this test
kit. Ninhydrin concentration studied was 0.5-3.5 % (w/v), and performed optimum absorbance of hydrindantin
at 1.0 %. Under the optimum time and ninhydrin of concentration, the cyanide
test kit standard color can be used to determine cyanide at the concentration
range of 0.5-6.0 ppm because the color of hydrindantin at concentration range
of cyanide 7.0-10.0 ppm was indistinguishable.
Penulis: Novy Lailatuz Zulfah,
Hermin Sulistyarti, Atikah
Kode Jurnal: jpkimiadd150024
